WB
WB analysis using CTHRC1 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CTHRC1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.ICC/IF
IF Staining of A375 using CTHRC1 Polyclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using CTHRC1 antibody at dilution of 1:200 and 488-Conjugated Goat Anti-Rabbit IgG(H+L) , 594-Phalloidin (red).IP
IP experiment of A375 using CTHRC1 Polyclonal antibody. IP result of anti-CTHRC1 (3ug; 1:500) with A375 cells lysate 3600ug.FC
FC experiment of A375 using CTHRC1 Polyclonal antibody. 1x10^6 A375 cells were intracellularly stained with 0.13 ug CTHRC1 Polyclonal antibody and 488-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.13 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | CTHRC1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CTHRC1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIPWB |
| WB:1:2000-1:16000 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC:0.13 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTHRC1 |
|---|---|
| Gene Full Name | collagen triple helix repeat containing 1 |
| Gene Summary | This locus encodes a protein that may play a role in the cellular response to arterial injury through involvement in vascular remodeling. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2012] |
| Alternative Names | collagen triple helix repeat containing 1 Collagen triple helix repeat-containing protein 1 Protein NMTC1 UNQ762/PRO1550 |
| Molecular Weight(MW) | 243 aa, 26 kDa(Observed band size:25-30 kDa) |
| Cellular Localization | Extracellular matrix,Secreted. |
WB
WB analysis using CTHRC1 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with CTHRC1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
ICC/IF
IF Staining of A375 using CTHRC1 Polyclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using CTHRC1 antibody at dilution of 1:200 and 488-Conjugated Goat Anti-Rabbit IgG(H+L) , 594-Phalloidin (red).
IP
IP experiment of A375 using CTHRC1 Polyclonal antibody. IP result of anti-CTHRC1 (3ug; 1:500) with A375 cells lysate 3600ug.
FC
FC experiment of A375 using CTHRC1 Polyclonal antibody. 1x10^6 A375 cells were intracellularly stained with 0.13 ug CTHRC1 Polyclonal antibody and 488-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.13 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:2000-1:16000 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC:0.13 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
