WB
Western blot analysis of CTHRC1 on different lysates with Rabbit anti-CTHRC1 antibody at 1/1,000 dilution.
Lysates/proteins at 20 ng/Lane.
Exposure time: 180s ;
Blocking: 5% NFDM/TBST, 1 hour at room temperature.
Primary antibody: CTHRC1 Mouse Monoclonal Antibody, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃.
Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.