IHC
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Mouse ortical bone tissues using IMACF1 | ACF7 Rabbit pAb with DAB staining| Product Name | MACF1 | ACF7 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human MACF1/ACF7 : 1-100/7388. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MACF1 |
|---|---|
| Gene Synonyms | ACF7 LIS9 MACF OFC4 ABP620 KIAA0754 Lnc-PMIF |
| Gene Full Name | microtubule actin crosslinking factor 1 |
| Gene Summary | This gene encodes a large protein containing numerous spectrin and leucine-rich repeat (LRR) domains. The encoded protein is a member of a family of proteins that form bridges between different cytoskeletal elements. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. Alternative splicing results in multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, May 2013] |
| Alternative Names | ABP620 Acf7 Aclp7 D830031N03Rik MACF mKIAA0465 mKIAA0754 MACF1_MOUSE Macf1 Actin cross-linking family 7 Kiaa0754 MACF1_RAT LIS9 Lnc-PMIF OFC4 microtubule actin crosslinking factor 1 actin cross-linking factor 620 kDa actin binding protein macrophin 1 trabeculin-alpha actin cross-linking family protein 7 postulated migration inhibitory factor |
| Molecular Weight(MW) | 839 kDa |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasm,Cytoskeleton,Golgi apparatus,Membrane,Microtubule. |
IHC
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse ortical bone tissues using IMACF1 | ACF7 Rabbit pAb with DAB staining| Application Notes | IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
