WB
Western blot analysis of OPN5 using anti-OPN5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OPN5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of OPN5 using anti-OPN5 antibody. OPN5 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-OPN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of OPN5 using anti-OPN5 antibody. OPN5 was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-OPN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of OPN5 using anti-OPN5 antibody. OPN5 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-OPN5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to OPN5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human OPN5. Human OPN5 shares 95.8% amino acid (aa) sequence identity with mouse OPN5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OPN5 |
|---|---|
| Gene Synonyms | PGR12 GPR136 GRP136 TMEM13 |
| Gene Full Name | opsin 5 |
| Gene Summary | Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this family member may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| Alternative Names | TMEM13 PGR12 opsin 5 NEUROPSIN GPR136 |
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Cell membrane,Membrane. |
WB
Western blot analysis of OPN5 using anti-OPN5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OPN5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of OPN5 using anti-OPN5 antibody. OPN5 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-OPN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of OPN5 using anti-OPN5 antibody. OPN5 was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-OPN5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of OPN5 using anti-OPN5 antibody. OPN5 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-OPN5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
