WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Oct-2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.WB
Western Blot analysis of various cells using Oct-2 Antibody.IHC
Human spleen was stained with anti-Oct-2 rabbit antibody.IHC
Mouse spleen was stained with anti-Oct-2 rabbit antibody.IHC
Rat spleen was stained with anti-Oct-2 rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Oct-2 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | Oct-2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| IHC:1:400-1:1000 WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | POU2F2 |
|---|---|
| Gene Synonyms | OCT2 OTF2 Oct-2 |
| Gene Full Name | POU class 2 homeobox 2 |
| Gene Summary | The protein encoded by this gene is a homeobox-containing transcription factor of the POU domain family. The encoded protein binds the octamer sequence 5'-ATTTGCAT-3', a common transcription factor binding site in immunoglobulin gene promoters. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | POU domain class 2 transcription factor 2 Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 Octamer-binding protein 2 Oct-2 Octamer-binding transcription factor 2 OTF-2 |
| Molecular Weight(MW) | 51 kDa(Observed band size: 65 kDa) |
| Cellular Localization | Nucleus |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Oct-2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
WB
Western Blot analysis of various cells using Oct-2 Antibody.
IHC
Human spleen was stained with anti-Oct-2 rabbit antibody.
IHC
Mouse spleen was stained with anti-Oct-2 rabbit antibody.
IHC
Rat spleen was stained with anti-Oct-2 rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Oct-2 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | IHC:1:400-1:1000 WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
