WB
Western blot analysis of OLR1 using anti-OLR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OLR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of OLR1 using anti-OLR1 antibody. OLR1 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-OLR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of Neuro-2a cells using anti-OLR1 antibody. Overlay histogram showing Neuro-2a cells stained with Rabbit polyclonal antibody to OLR1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLR1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to OLR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived rat LOX 1/Olr1 recombinant protein (Position: Q55-Q364). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Olr1 |
|---|---|
| Gene Synonyms | LOX-1 Oldr1 Oldlr1 |
| Gene Full Name | oxidized low density lipoprotein receptor 1 |
| Gene Summary | Predicted to enable identical protein binding activity and low-density lipoprotein particle receptor activity. Involved in several processes, including lipoprotein metabolic process; negative regulation of miRNA transcription; and response to hydrogen peroxide. Predicted to be located in nucleoplasm. Predicted to be part of receptor complex. Predicted to be active in plasma membrane. Biomarker of end stage renal disease and hypertension. Human ortholog(s) of this gene implicated in cerebral amyloid angiopathy; coronary artery disease; and myocardial infarction. Orthologous to human OLR1 (oxidized low density lipoprotein receptor 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CLEC8A hLOX 1 Lectin like oxLDL receptor 1 LOX 1 LOX1 OLR1 Ox LDL receptor 1 SCARE1 |
| Molecular Weight(MW) | 42 kDa(Observed band size:52 kDa) |
| Cellular Localization | Cell membrane,Membrane,Secreted. |
WB
Western blot analysis of OLR1 using anti-OLR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OLR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of OLR1 using anti-OLR1 antibody. OLR1 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-OLR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of Neuro-2a cells using anti-OLR1 antibody. Overlay histogram showing Neuro-2a cells stained with Rabbit polyclonal antibody to OLR1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLR1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
