WB
Western blot analysis of Phospho-TDP43 (S409, S410) on different lysates with Rabbit anti-Phospho-TDP43 (S409, S410) antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.WB
Western blot analysis of Phospho-TDP43 (S409, S410) on different lysates with Rabbit anti-Phospho-TDP43 (S409, S410) antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Stomach. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Testis. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Stomach. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Product Name | Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser409 and Ser410 of Human TDP43. |
| Modification | p-S409/S410 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:5000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TARDBP |
|---|---|
| Gene Synonyms | ALS10 TDP-43 |
| Gene Full Name | TAR DNA binding protein |
| Gene Summary | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008] |
| Alternative Names | ALS10 antibody OTTHUMP00000002171 antibody OTTHUMP00000002172 antibody OTTHUMP00000002173 antibody TADBP_HUMAN antibody TAR DNA binding protein 43 antibody TAR DNA binding protein antibody TAR DNA-binding protein 43 antibody TARDBP antibody TDP 43 antibody TDP-43 antibody TDP43 antibody |
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm, Mitochondrion, Nucleus. |
WB
Western blot analysis of Phospho-TDP43 (S409, S410) on different lysates with Rabbit anti-Phospho-TDP43 (S409, S410) antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
WB
Western blot analysis of Phospho-TDP43 (S409, S410) on different lysates with Rabbit anti-Phospho-TDP43 (S409, S410) antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Stomach. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Testis. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Stomach. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Phospho-TDP43 (S409, S410) Rabbit Polyclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.| Application Notes | WB:1:5000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
