WB
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody at 1/5,000 dilution. Lysates/proteins at 30 ng/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | VDAC1 / 2 / 3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human VDAC2 aa 1-294. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | VDAC1/VDAC2/VDAC3 |
|---|---|
| Gene Synonyms | PORIN VDAC-1/VDAC-3 HD-VDAC3 |
| Gene Full Name | voltage dependent anion channel 1/voltage dependent anion channel 2/voltage dependent anion channel 3 |
| Gene Summary | This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010] |
| Alternative Names | hVDAC1 antibody MGC111064 antibody Mithocondrial porin antibody OPM2 yeast human complement of antibody Outer mitochondrial membrane protein porin 1 antibody Plasmalemmal porin antibody Porin 31 HL antibody Porin 31 HM antibody Porin 31HL antibody Porin 31HM antibody Porin antibody VDAC 1 antibody VDAC antibody VDAC-1 antibody Vdac1 antibody VDAC1_HUMAN antibody Voltage Dependent Anion Channel 1 antibody voltage dependent anion selective channel protein 1 antibody Voltage-dependent anion-selective channel protein 1 antibody B-36 vdac antibody FLJ23841 antibody hVDAC2 antibody OTTHUMP00000019878 antibody OTTHUMP00000019879 antibody OTTHUMP00000019880 antibody Outer mitochondrial membrane protein porin 2 antibody POR antibody Porin-2 antibody VDAC 2 antibody VDAC-2 antibody VDAC2 antibody VDAC2_HUMAN antibody Vdac6 antibody Voltage dependent anion channel 2 antibody Voltage dependent anion selective channel protein 2 antibody Voltage-depend |
| Molecular Weight(MW) | 31 kDa(Observed band size:35 kDa) |
| Cellular Localization | Mitochondrion outer membrane, Membrane. |
WB
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of VDAC1 / 2 / 3 on different lysates with Rabbit anti-VDAC1 / 2 / 3 antibody at 1/5,000 dilution. Lysates/proteins at 30 ng/Lane. Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
