WB
Western blot analysis of ECSIT using anti-ECSIT antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ECSIT antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ECSIT using anti-ECSIT antibody. ECSIT was detected in a paraffin-embedded section of human bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ECSIT Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of ECSIT using anti-ECSIT antibody. ECSIT was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-ECSIT Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-ECSIT antibody. Overlay histogram showing HepG2 cells stained with Rabbit polyclonal antibody to ECSIT (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ECSIT Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ECSIT |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ECSIT recombinant protein (Position: E83-S339). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ECSIT |
|---|---|
| Gene Synonyms | SITPEC |
| Gene Full Name | ECSIT signaling integrator |
| Gene Summary | Enables molecular adaptor activity. Involved in regulation of oxidoreductase activity; regulation of protein complex stability; and toll-like receptor 4 signaling pathway. Located in cytosol; mitochondrion; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ECSIT Protein SITPEC SITPEC |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Cytoplasm,Mitochondrion,Nucleus. |
WB
Western blot analysis of ECSIT using anti-ECSIT antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ECSIT antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ECSIT using anti-ECSIT antibody. ECSIT was detected in a paraffin-embedded section of human bladder adenosquamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ECSIT Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ECSIT using anti-ECSIT antibody. ECSIT was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-ECSIT Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-ECSIT antibody. Overlay histogram showing HepG2 cells stained with Rabbit polyclonal antibody to ECSIT (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ECSIT Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
