Application
Immunohistochemistry of ECSIT in mouse heart cells with ECSIT antibody at 2 ug/ml.Application
Western blot analysis of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.| Product Name | ECSIT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ECSITActivation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways. |
| Immunogen | Human ECSIT (C-Terminus) Peptide |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ECSIT |
|---|---|
| Target | ECSIT |
| Tissue Specificity | This antibody is specific for ECSIT |
Application
Immunohistochemistry of ECSIT in mouse heart cells with ECSIT antibody at 2 ug/ml.
Application
Western blot analysis of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.| Positive Control | Blocking Peptide: Cat. No. GTX85081-PEP - ECSIT Peptide Lysates: Cat. No. GTX27916 - Human Heart Tissue LysateCat. No. GTX27932 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
