WB
Western blot analysis of various lysates using PCIF1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.IHC
Immunohistochemistry analysis of paraffin embedded Rat brain using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse spinal cord using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.IP
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg PCIF1 antibody. Western blot was performed from the immunoprecipitate using PCIF1 antibody at a dilution of 1:1000.| Product Name | PCIF1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500 - 1:2000 IHC:1:50 - 1:100 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCIF1 |
|---|---|
| Gene Synonyms | CAPAM MT-A70 hCAPAM hPCIF1 C20orf67 PPP1R121 |
| Gene Full Name | phosphorylated CTD interacting factor 1 |
| Gene Summary | Enables RNA polymerase II C-terminal domain phosphoserine binding activity; S-adenosyl-L-methionine binding activity; and mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity. Involved in mRNA processing; negative regulation of translation; and positive regulation of translation. Located in intercellular bridge; microtubule cytoskeleton; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CAPAM hCAPAM hPCIF1 C20orf67 PPP1R121 PCIF1 |
| Molecular Weight(MW) | 81kDa |
| Cellular Localization | intercellular bridge, microtubule cytoskeleton, nucleoplasm, nucleus. |
WB
Western blot analysis of various lysates using PCIF1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
IHC
Immunohistochemistry analysis of paraffin embedded Rat brain using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse spinal cord using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
IP
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg PCIF1 antibody. Western blot was performed from the immunoprecipitate using PCIF1 antibody at a dilution of 1:1000.| Application Notes | WB:1:500 - 1:2000 IHC:1:50 - 1:100 IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
