WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was incubated with anti-CUL-3 antibody at a dilution of 1:2000. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Mouse testis was stained with anti-CUL-3 Rabbit antibody.IHC
Human testis was stained with anti-CUL-3 Rabbit antibody.| Product Name | CUL-3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CUL3 |
|---|---|
| Gene Synonyms | CUL-3 PHA2E NEDAUS |
| Gene Full Name | cullin 3 |
| Gene Summary | This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Alternative Names |
CUL3 KIAA0617 Cullin-3 CUL-3 |
| Molecular Weight(MW) | 89 kDa |
| Cellular Localization | Nucleus . Golgi apparatus . Cell projection , cilium , flagellum . Cytoplasm , cytoskeleton , spindle . Cytoplasm. Cytoplasm , cytoskeleton , microtubule organizing center , centrosome . Cytoplasm , cytoskeleton , spindle pole . Detected along the length of the sperm flagellum and in the cytoplasm of the germ cells (PubMed:28395323) . Predominantly found in the nucleus in interphase cells , found at the centrosome at late G2 or prophase , starts accumulating at the spindle poles in prometaphase and stays on the spindle poles and the mitotic spindle at metaphase (PubMed:23213400) . . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was incubated with anti-CUL-3 antibody at a dilution of 1:2000. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Mouse testis was stained with anti-CUL-3 Rabbit antibody.
IHC
Human testis was stained with anti-CUL-3 Rabbit antibody.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
