WB
25 ug total protein per lane of various lysates (see on figure) probed with RBX1 monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBX1 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP-conjugated secondary antibody and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBX1 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP-conjugated secondary antibody and DAB (C-0010) staining.IHC
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBX1 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP-conjugated secondary antibody and DAB (C-0010) staining.ICC/IF
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RBX1) monoclonal Antibody, unconjugated 1:200, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min . DAPI (blue) was used to stain the cell nuclei.FC
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-RBX1 antibody (1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 : 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.| Product Name | RBX1 Recombinant Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human RBX1 : 60-108/108. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 ICC/IF:1:100-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RBX1 |
|---|---|
| Gene Synonyms | ROC1 RNF75 BA554C12.1 |
| Gene Full Name | ring-box 1 |
| Gene Summary | This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010] |
| Alternative Names | BA554C12.1 RNF75 ROC1 1500002P15Rik RBX1_HUMAN RBX1 E3 ubiquitin-protein transferase RBX1 Protein ZYP RING finger protein 75 RING-box protein 1 (Rbx1) Regulator of cullins 1 (ROC1) 2.3.2.27 2.3.2.32 RBX1_MOUSE ring-box 1 regulator of cullins 1 |
| Molecular Weight(MW) | 12 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
25 ug total protein per lane of various lysates (see on figure) probed with RBX1 monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBX1 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP-conjugated secondary antibody and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBX1 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP-conjugated secondary antibody and DAB (C-0010) staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RBX1 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP-conjugated secondary antibody and DAB (C-0010) staining.
ICC/IF
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RBX1) monoclonal Antibody, unconjugated 1:200, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min . DAPI (blue) was used to stain the cell nuclei.
FC
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-RBX1 antibody (1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 : 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 ICC/IF:1:100-1:500 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
