WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-MDR1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human liver was stained with anti-MDR1 rabbit antibody.IF-P
Fluorescence immunohistochemical analysis of Human liver.| Product Name | MDR1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ABCB1 |
|---|---|
| Gene Synonyms | CLCS MDR1 P-GP PGY1 ABC20 CD243 ENPAT GP170 p-170 |
| Gene Full Name | ATP binding cassette subfamily B member 1 |
| Gene Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017] |
| Alternative Names | ABC20 ABCB1 ATP binding cassette sub family B MDR/TAP member 1 ATP-binding cassette sub-family B member 1 CD243 CLCS Colchicin sensitivity Doxorubicin resistance GP170 MDR1 MDR1_HUMAN Multidrug resistance 1 Multidrug resistance protein 1 P glycoprotein 1 P gp P-glycoprotein 1 PGY1 P Glycoprotein |
| Molecular Weight(MW) | 141 kDa(Observed band size: 140-180 kDa) |
| Cellular Localization | Membrane. |
WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-MDR1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human liver was stained with anti-MDR1 rabbit antibody.
IF-P
Fluorescence immunohistochemical analysis of Human liver.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
