WB
Western Blot of Rabbit anti-Ngp antibody. Lane 1: MW ladder (opal pre-stained) . Lane 2: 32D lysate. Load: 10 µg per lane. Primary antibody: Ngp antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody at 1:70,000 for 45 min at RT. Block: BlockOut overnight at 4°C. Predicted/Observed size: 17, 20 kDa for Ngp. Higher banding due to glycosylation. ~125 kDa non-specific.| Product Name | Anti-Ngp (neutrophilic granule protein) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A recombinant protein of mouse Ngp protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:2000 |
|
| Species Reactivity | Mouse |
| Purification | Affinity purified |
| Gene Symbol | Ngp |
|---|---|
| Gene Synonyms | bectenecin |
| Gene Full Name | neutrophilic granule protein |
| Gene Summary | Enables cysteine-type endopeptidase inhibitor activity. Involved in negative regulation of angiogenesis and negative regulation of lymphangiogenesis. Located in cytoplasmic vesicle and extracellular region. Is expressed in liver and liver lobe. [provided by Alliance of Genome Resources, Jun 2026] |
| Alternative Names | clone B6 cystatin-like protein myeloid bactenecin protein myeloid granule protein myeloid secondary granule protein |
| Molecular Weight(MW) | 17, 20 kDa |
WB
Western Blot of Rabbit anti-Ngp antibody. Lane 1: MW ladder (opal pre-stained) . Lane 2: 32D lysate. Load: 10 µg per lane. Primary antibody: Ngp antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody at 1:70,000 for 45 min at RT. Block: BlockOut overnight at 4°C. Predicted/Observed size: 17, 20 kDa for Ngp. Higher banding due to glycosylation. ~125 kDa non-specific.| Application Notes | WB:1:1000 ICC/IF:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Storage Buffer | 0.01% (w/v) Sodium Azide,0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
