WB
Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using ATP1A1 mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.FC
Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to ATP1A1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human ATP1A1 (AA: 153-288) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500 - 1:2000 IHC:1:200 - 1:1000 ICC/IF:1:200 - 1:1000 FC:1:200 - 1:400 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP1A1 |
|---|---|
| Gene Synonyms | CMT2DD HOMGSMR2 |
| Gene Full Name | ATPase Na+/K+ transporting subunit alpha 1 |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Alternative Names | CMT2DD HOMGSMR2 |
| Molecular Weight(MW) | 112.8 kDa |
| Cellular Localization | Cell membrane,Cell projection,Membrane. |
WB
Western blot analysis using ATP1A1 mouse mAb against Hela (1), HepG2 (2), and A431 (3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using ATP1A1 mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher.
FC
Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:500 - 1:2000 IHC:1:200 - 1:1000 ICC/IF:1:200 - 1:1000 FC:1:200 - 1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
