WB
Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate. All lanes: ERVFRD-1 antibody at 1:1000. Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.ICC/IF
Immunofluorescence staining of Hela cell with Rabbit anti-ERVFRD-1 Polyclonal antibody at 1:160, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-ERVFRD-1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Syncytin-2 protein (16-250AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC/IF:1:100-1:300 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ERVFRD-1 |
|---|---|
| Gene Synonyms | envFRD UNQ6191 ERVFRDE1 GLLL6191 HERV-FRD HERV-W/FRD |
| Gene Full Name | endogenous retrovirus group FRD member 1, envelope |
| Gene Summary | Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of a human endogenous retrovirus provirus on chromosome 6 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product plays a major role in placental development and trophoblast fusion. The protein has the characteristics of a typical retroviral envelope protein, including a cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. [provided by RefSeq, Jun 2012] |
| Alternative Names | EFRD1_HUMAN antibody Endogenous retrovirus group FRD member 1 antibody Envelope polyprotein antibody ERVFRD-1 antibody FLJ41944 antibody FLJ90611 antibody GLLL6191 antibody HERV FRD 6p24.1 provirus ancestral Env polyprotein antibody HERV FRD antibody HERV FRD provirus ancestral Env polyprotein antibody HERV-FRD antibody HERVFRD antibody HERVFRD provirus ancestral Env polyprotein antibody MGC87585 antibody SU antibody Syncytin 2 antibody Syncytin B antibody Syncytin-2 antibody TM antibody Transmembrane protein antibody UNQ6191 antibody |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane,Membrane,Viral envelope protein,Virion. |
WB
Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate. All lanes: ERVFRD-1 antibody at 1:1000. Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
ICC/IF
Immunofluorescence staining of Hela cell with Rabbit anti-ERVFRD-1 Polyclonal antibody at 1:160, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:5000 ICC/IF:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
