WB
Western Blot. All lanes: NDUFB8 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/40000 dilution. Exposure time：20s.IHC
IHC image of NDUFB8 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.| Product Name | NDUFB8 Recombinant Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human NDUFB8. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFB8 |
|---|---|
| Gene Synonyms | ASHI CI-ASHI MC1DN32 |
| Gene Full Name | NADH:ubiquinone oxidoreductase subunit B8 |
| Gene Summary | Predicted to enable NADH dehydrogenase (ubiquinone) activity. Predicted to be involved in mitochondrial electron transport, NADH to ubiquinone and proton motive force-driven mitochondrial ATP synthesis. Located in mitochondrial inner membrane. Part of respiratory chain complex I. Implicated in nuclear type mitochondrial complex I deficiency 32. Biomarker of Alzheimer's disease and Parkinson's disease. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ASHI antibody CI-ASHI antibody Complex I ASHI subunit antibody Complex I-ASHI antibody mitochondrial antibody NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody NADH-ubiquinone oxidoreductase ASHI subunit antibody NDUB8_HUMAN antibody NDUFB8 antibody |
| Molecular Weight(MW) | 22 kDa(Observed band size:19 kDa) |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane. |
WB
Western Blot. All lanes: NDUFB8 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/40000 dilution. Exposure time：20s.
IHC
IHC image of NDUFB8 Recombinant Monoclonal Antibody diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
