WB
Western Blot. All lanes: NDUFB8 antibody at 2μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
IHC image of NDUFB8 Antibody diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.| Product Name | Rabbit anti-NDUFB8 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial protein (29-132AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:5000 IHC:1:200-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | NDUFB8 |
|---|---|
| Gene Synonyms | ASHI CI-ASHI MC1DN32 |
| Gene Full Name | NADH:ubiquinone oxidoreductase subunit B8 |
| Gene Summary | Predicted to enable NADH dehydrogenase (ubiquinone) activity. Predicted to be involved in mitochondrial electron transport, NADH to ubiquinone and proton motive force-driven mitochondrial ATP synthesis. Located in mitochondrial inner membrane. Part of respiratory chain complex I. Implicated in nuclear type mitochondrial complex I deficiency 32. Biomarker of Alzheimer's disease and Parkinson's disease. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | NDUFB8 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial Complex I-ASHI CI-ASHI NADH-ubiquinone oxidoreductase ASHI subunit |
| Molecular Weight(MW) | 22, 21, 19 kDa(Observed band size: 22 kDa) |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane. |
WB
Western Blot. All lanes: NDUFB8 antibody at 2μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of NDUFB8 Antibody diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.| Application Notes | WB:1:500-1:5000 IHC:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. |
