WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IκB-α (Phospho Ser36) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human colon was stained with anti-IκB-α (Phospho Ser36) Rabbit antibodyIF-P
Fluorescence multiplex immunohistochemical analysis of Human kidney.| Product Name | IκB-α (Phospho Ser36) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Modification | p-Ser36 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:2000-1:10000 IHC:1:500-1:1000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NFKBIA |
|---|---|
| Gene Synonyms | IKBA MAD-3 NFKBI EDAID2 |
| Gene Full Name | NFKB inhibitor alpha |
| Gene Summary | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Alternative Names |
NFKBIA IKBA MAD3 NFKBI NF-kappa-B inhibitor alpha I-kappa-B-alpha IkB-alpha IkappaBalpha Major histocompatibility complex enhancer-binding protein MAD3 |
| Molecular Weight(MW) | 36 kDa(Observed band size: 39 kDa) |
| Cellular Localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IκB-α (Phospho Ser36) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human colon was stained with anti-IκB-α (Phospho Ser36) Rabbit antibody
IF-P
Fluorescence multiplex immunohistochemical analysis of Human kidney.| Application Notes | WB:1:2000-1:10000 IHC:1:500-1:1000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
