WB
WB analysis of PC-3 using ZWILCH Polyclonal antibody. PC-3 cells were subjected to SDS PAGE followed by western blot with ZWILCH Antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
IHC staining of human colon cancer using ZWILCH Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using ZWILCH antibody at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | ZWILCH Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ZWILCH protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZWILCH |
|---|---|
| Gene Synonyms | KNTC1AP hZwilch |
| Gene Full Name | zwilch kinetochore protein |
| Gene Summary | Involved in mitotic spindle assembly checkpoint signaling and protein localization to kinetochore. Located in kinetochore. Part of RZZ complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Protein zwilch homolog KNTC1AP hZwilch |
| Molecular Weight(MW) | 67 kDa(Observed band size: ~60 kDa) |
| Cellular Localization | Centromere,Chromosome,Kinetochore. |
WB
WB analysis of PC-3 using ZWILCH Polyclonal antibody. PC-3 cells were subjected to SDS PAGE followed by western blot with ZWILCH Antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human colon cancer using ZWILCH Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using ZWILCH antibody at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:1000 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
