WB
Western blot analysis of UQCRC2 using anti-UQCRC2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were traUQCRC2erred to a membrane. Then the membrane was incubated with rabbit anti-UQCRC2 antigen affinity purified polyclonal antibody at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The siUQCRC2 is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of UQCRC2 using anti-UQCRC2 antibody. UQCRC2 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-UQCRC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of UQCRC2 using anti-UQCRC2 antibody. UQCRC2 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-UQCRC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of UQCRC2 using anti-UQCRC2 antibody. UQCRC2 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-UQCRC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of UQCRC2 using anti-UQCRC2 antibody. UQCRC2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-UQCRC2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of UQCRC2 using anti-UQCRC2 antibody in Hela whole cell lysate. Western blot analysis of UQCRC2 using anti- UQCRC2 antibody. After electrophoresis, proteins were traUQCRC2erred to a membrane. Then the membrane was incubated with rabbit anti- UQCRC2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The siUQCRC2 is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to UQCRC2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human UQCRC2 recombinant protein (Position: L52-K436). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000-1:10000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UQCRC2 |
|---|---|
| Gene Synonyms | QCR2 UQCR2 MC3DN5 |
| Gene Full Name | ubiquinol-cytochrome c reductase core protein 2 |
| Gene Summary | The protein encoded by this gene is located in the mitochondrion, where it is part of the ubiquinol-cytochrome c reductase complex (also known as complex III). This complex constitutes a part of the mitochondrial respiratory chain. Defects in this gene are a cause of mitochondrial complex III deficiency nuclear type 5. [provided by RefSeq, Jul 2015] |
| Alternative Names | UQCR2 Ubiquinol-cytochrome-c reductase complex core protein 2 QCR2 Cytochrome b-c1 complex subunit 2 mitochondrial Core protein II |
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane. |
WB
Western blot analysis of UQCRC2 using anti-UQCRC2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were traUQCRC2erred to a membrane. Then the membrane was incubated with rabbit anti-UQCRC2 antigen affinity purified polyclonal antibody at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The siUQCRC2 is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of UQCRC2 using anti-UQCRC2 antibody. UQCRC2 was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-UQCRC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of UQCRC2 using anti-UQCRC2 antibody. UQCRC2 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-UQCRC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of UQCRC2 using anti-UQCRC2 antibody. UQCRC2 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-UQCRC2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of UQCRC2 using anti-UQCRC2 antibody. UQCRC2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-UQCRC2 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of UQCRC2 using anti-UQCRC2 antibody in Hela whole cell lysate. Western blot analysis of UQCRC2 using anti- UQCRC2 antibody. After electrophoresis, proteins were traUQCRC2erred to a membrane. Then the membrane was incubated with rabbit anti- UQCRC2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The siUQCRC2 is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:2000-1:10000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
