WB
Western Blot. Positive WB detected in: Mouse heart tissue. All lanes: SEMA5B antibody at 1μg/ml. Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human testis tissue using SEMA5B Antibody at dilution of 1:100.ICC/IF
Immunofluorescence staining of Hela cells with SEMA5B Antibody at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-SEMA5B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Semaphorin-5B protein (845-1013AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SEMA5B |
|---|---|
| Gene Synonyms | SemG SEMAG |
| Gene Full Name | semaphorin 5B |
| Gene Summary | This gene encodes a member of the semaphorin protein family which regulates axon growth during development of the nervous system. The encoded protein has a characteristic Sema domain near the N-terminus, through which semaphorins bind to plexin, and five thrombospondin type 1 repeats in the C-terminal region of the protein. The protein product may be cleaved and exist as a secreted molecule (PMID: 19463192). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| Alternative Names | FLJ10372 antibody KIAA1445 antibody SEM5B_HUMAN antibody SEMA5B antibody SEMAG antibody Semaphorin-5B antibody SemG antibody |
| Molecular Weight(MW) | 126, 120, 124, 132 kDa(Observed band size: 126 kDa) |
| Cellular Localization | Membrane. |
WB
Western Blot. Positive WB detected in: Mouse heart tissue. All lanes: SEMA5B antibody at 1μg/ml. Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human testis tissue using SEMA5B Antibody at dilution of 1:100.
ICC/IF
Immunofluorescence staining of Hela cells with SEMA5B Antibody at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. |
