IHC
IHC image of CHAF1A Antibody diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.IHC
IHC image of CHAF1A Antibody diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.| Product Name | Rabbit anti-CHAF1A Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Chromatin assembly factor 1 subunit A protein (840-904AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:200-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CHAF1A |
|---|---|
| Gene Synonyms | CAF1 P150 CAF-1 CAF1B CAF1P150 |
| Gene Full Name | chromatin assembly factor 1 subunit A |
| Gene Summary | Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008] |
| Alternative Names | CAF 1 antibody CAF 1 subunit A antibody CAF antibody CAF I 150 kDa subunit antibody CAF I p150 antibody CAF-1 subunit A antibody CAF-I 150 kDa subunit antibody CAF-I p150 antibody CAF1 antibody CAF1A_HUMAN antibody CAF1B antibody CAF1P150 antibody CHAF1A antibody Chromatin assembly factor 1 subunit A antibody Chromatin Assembly Factor 1 Subunit A p150 antibody Chromatin assembly factor I (150 kDa) antibody Chromatin assembly factor I 150 kDa antibody Chromatin assembly factor I p150 subunit antibody DCAF1 antibody hp150 antibody MGC71229 antibody P150 antibody |
| Cellular Localization | Nucleus. |
IHC
IHC image of CHAF1A Antibody diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
IHC image of CHAF1A Antibody diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.| Application Notes | IHC:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. |
