WB
Western blot analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TORC1/CRTC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody and anti-Alpha Tubulin antibody. TORC1/CRTC1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-TORC1/CRTC1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.FC
Flow Cytometry analysis of MCF-7 cells using anti-TORC1/CRTC1 antibody. Overlay histogram showing MCF-7 cells stained with Anti-TORC1/CRTC1 Antibody (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TORC1/CRTC1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to TORC1/CRTC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human TORC1/CRTC1 recombinant protein (Position: E30-E568). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRTC1 |
|---|---|
| Gene Synonyms | MAML2 MECT1 Mam-2 TORC1 TORC-1 WAMTP1 |
| Gene Full Name | CREB regulated transcription coactivator 1 |
| Gene Summary | Enables cAMP response element binding protein binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in cytosol and nuclear body. [provided by Alliance of Genome Resources, Jun 2026] |
| Alternative Names | CRTC1 KIAA0616 MECT1 TORC 1 TORC1 TORC1/CRTC1 Transducer of CREB protein 1 WAMTP1 |
| Molecular Weight(MW) | 67 kDa(Observed band size: 78 kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western blot analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TORC1/CRTC1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of TORC1/CRTC1 using anti-TORC1/CRTC1 antibody and anti-Alpha Tubulin antibody. TORC1/CRTC1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-TORC1/CRTC1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
FC
Flow Cytometry analysis of MCF-7 cells using anti-TORC1/CRTC1 antibody. Overlay histogram showing MCF-7 cells stained with Anti-TORC1/CRTC1 Antibody (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TORC1/CRTC1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
