WB
Western blotting analysis using ASRGL1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ASRGL1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively.
FC
Validation of ASRGL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ASRGL1 antibody and analyzed using BD flow cytometer.