WB
Western blot analysis of extracts from Mouse lung tissue using Collagen VI Rabbit mAb at 1:1000.WB
Western blot analysis of extracts from Human placenta tissue using Collagen VI Rabbit mAb at 1:1000.ICC/IF
Immunofluorescence analysis of HeLa cells labelling Collagen VI with Collagen VI Rabbit mAb. The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with Collagen VI Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Product Name | Collagen VI Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of human Collagen VI. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL6A1 |
|---|---|
| Gene Synonyms | OPLL BTHLM1 UCHMD1 BTHLM1A UCHMD1A |
| Gene Full Name | collagen type VI alpha 1 chain |
| Gene Summary | The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008] |
| Alternative Names | OPLL BTHLM1 UCHMD1 |
| Molecular Weight(MW) | 109 kDa(Observed band size: 147 kDa) |
| Cellular Localization | Extracellular matrix,Secreted. |
WB
Western blot analysis of extracts from Mouse lung tissue using Collagen VI Rabbit mAb at 1:1000.
WB
Western blot analysis of extracts from Human placenta tissue using Collagen VI Rabbit mAb at 1:1000.
ICC/IF
Immunofluorescence analysis of HeLa cells labelling Collagen VI with Collagen VI Rabbit mAb. The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with Collagen VI Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Application Notes | WB:1:1000 ICC/IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C.Stable for 12 months from date of receipt. |
| Storage Buffer | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
