WB
Western blot analysis of GDNF using anti-GDNF antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GDNF antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of GDNF using anti-GDNF antibody. GDNF was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-GDNF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of GDNF using anti-GDNF antibody. GDNF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GDNF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to GDNF |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human GDNF, different from the related mouse sequence by three amino acids and rat sequence by two amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GDNF |
|---|---|
| Gene Synonyms | ATF ATF1 ATF2 HSCR3 HFB1-GDNF |
| Gene Full Name | glial cell derived neurotrophic factor |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016] |
| Alternative Names | GDNF HFB1 GDNF hGDNF ATF ATF1 ATF2 HFB1-GDNF HSCR3 |
| Molecular Weight(MW) | 24 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of GDNF using anti-GDNF antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GDNF antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of GDNF using anti-GDNF antibody. GDNF was detected in a paraffin-embedded section of human stomach cancer tissue. The tissue section was incubated with rabbit anti-GDNF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of GDNF using anti-GDNF antibody. GDNF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-GDNF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
