WB
Western blot analysis of RAB27A on different lysates with Rabbit anti-RAB27A antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; Negative control : Raji. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: HA724422, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Malignant melanoma. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: RAB27A Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Colon. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: RAB27A Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Colon. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: RAB27A Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.FC
Application: Flow Cytometry (Intra). Species: Rat. Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated)). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: RAB27A Recombinant Rabbit Monoclonal Antibody(1/1,000) (Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.IP
Immunoprecipitation (IP). RAB27A was immunoprecipitated in 0.2 mg K-562 (Human chronic myelogenous leukemia cells) cell lysate with RAB27A Recombinant Rabbit Monoclonal Antibodyat 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using RAB27A Recombinant Rabbit Monoclonal Antibodyat 1/5,000 dilution. Alpaca anti-Rabbit IgG Fc, Recombinant VHH monoclonal Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input). Lane 2: RAB27A Recombinant Rabbit Monoclonal AntibodyIP in K-562 cell lysate. Lane 3: Rabbit IgG instead of RAB27A Recombinant Rabbit Monoclonal Antibodyin K-562 cell lysate. Exposure time: 14 seconds. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary dilution: RAB27A Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, 2 hours at room temperature.| Product Name | RAB27A Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human RAB27A aa 1-221. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCIPWB |
| WB:1:5000 IHC:1:200 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAB27A |
|---|---|
| Gene Synonyms | GS2 RAM RAB27 HsT18676 |
| Gene Full Name | RAB27A, member RAS oncogene family |
| Gene Summary | The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | GS2 antibody GTP-binding protein Ram antibody HsT18676 antibody MGC117246 antibody Mutant Ras related protein Rab-27A antibody Rab-27 antibody RAB-27A antibody RAB27 antibody RAB27A antibody RAB27A member RAS oncogene family antibody RAM antibody Ras-related protein Rab-27A antibody Ras-related protein Rab27A antibody RB27A_HUMAN antibody |
| Molecular Weight(MW) | 25 kDa(Observed band size: 27 kDa) |
| Cellular Localization | Membrane, Melanosome, Late endosome, Lysosome. |
WB
Western blot analysis of RAB27A on different lysates with Rabbit anti-RAB27A antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; Negative control : Raji. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: HA724422, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Malignant melanoma. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: RAB27A Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Colon. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: RAB27A Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Colon. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: RAB27A Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
FC
Application: Flow Cytometry (Intra). Species: Rat. Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated)). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: RAB27A Recombinant Rabbit Monoclonal Antibody(1/1,000) (Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.
IP
Immunoprecipitation (IP). RAB27A was immunoprecipitated in 0.2 mg K-562 (Human chronic myelogenous leukemia cells) cell lysate with RAB27A Recombinant Rabbit Monoclonal Antibodyat 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using RAB27A Recombinant Rabbit Monoclonal Antibodyat 1/5,000 dilution. Alpaca anti-Rabbit IgG Fc, Recombinant VHH monoclonal Antibody at 1/50,000 dilution was used for 1 hour at room temperature. Lane 1: K-562 cell lysate (input). Lane 2: RAB27A Recombinant Rabbit Monoclonal AntibodyIP in K-562 cell lysate. Lane 3: Rabbit IgG instead of RAB27A Recombinant Rabbit Monoclonal Antibodyin K-562 cell lysate. Exposure time: 14 seconds. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary dilution: RAB27A Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, 2 hours at room temperature.| Application Notes | WB:1:5000 IHC:1:200 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
