WB
WB analysis using TNFSF11/RANKL Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with RANKL antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
WB analysis of DC2.4 using TNFSF11/RANKL Monoclonal antibody. DC2.4 cells were subjected to SDS PAGE followed by western blot with RANKL antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
WB analysis of human spleen using TNFSF11/RANKL Monoclonal antibody. human spleen tissue were subjected to SDS PAGE followed by western blot with RANKL antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.ICC/IF
IF Staining of MCF-7 using TNFSF11/RANKL Monoclonal antibody. Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RANKL antibody at dilution of 1:800 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Product Name | TNFSF11/RANKL Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RANKL protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:2000-1:10000 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | TNFSF11 |
|---|---|
| Gene Synonyms | ODF OPGL sOdf CD254 OPTB2 RANKL TNLG6B TRANCE hRANKL2 |
| Gene Full Name | TNF superfamily member 11 |
| Gene Summary | This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dendritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. [provided by RefSeq, Mar 2026] |
| Alternative Names | TNFSF11 3F2E1 CD 254 CD254 Receptor activator of nuclear factor kappa-B ligand |
| Molecular Weight(MW) | 317 aa, 35 kDa(Observed band size: 35-38 kDa) |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane,Secreted. |
WB
WB analysis using TNFSF11/RANKL Monoclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with RANKL antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
WB analysis of DC2.4 using TNFSF11/RANKL Monoclonal antibody. DC2.4 cells were subjected to SDS PAGE followed by western blot with RANKL antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
WB analysis of human spleen using TNFSF11/RANKL Monoclonal antibody. human spleen tissue were subjected to SDS PAGE followed by western blot with RANKL antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
ICC/IF
IF Staining of MCF-7 using TNFSF11/RANKL Monoclonal antibody. Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RANKL antibody at dilution of 1:800 and 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:2000-1:10000 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
