WB
Western blot analysis of PCK1 on different lysates with Rabbit anti-PCK1 antibody at 1/5,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 40 seconds; Low expression: spleen. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: PCK1 Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.| Product Name | PCK1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human PCK1 aa 573-622. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PCK1 |
|---|---|
| Gene Synonyms | PCKDC PEPCK1 PEPCKC PEPCK-C |
| Gene Full Name | phosphoenolpyruvate carboxykinase 1 |
| Gene Summary | This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008] |
| Alternative Names | cytosolic [GTP] antibody GTP antibody PCK1 antibody PEP carboxykinase antibody PEPCK-C antibody PEPCK1 antibody PEPCKC antibody Phosphoenolpyruvate carboxykinase 1 (soluble) antibody Phosphoenolpyruvate carboxykinase 1 antibody Phosphoenolpyruvate carboxykinase antibody Phosphoenolpyruvate carboxykinase cytosolic [GTP] antibody Phosphoenolpyruvate carboxykinase cytosolic antibody Phosphoenolpyruvate carboxylase antibody Phosphopyruvate carboxylase antibody |
| Molecular Weight(MW) | 69 kDa |
| Cellular Localization | Cytoplasm, cytosol, Endoplasmic reticulum. |
WB
Western blot analysis of PCK1 on different lysates with Rabbit anti-PCK1 antibody at 1/5,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 40 seconds; Low expression: spleen. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: PCK1 Recombinant Rabbit Monoclonal Antibody, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
