IF-P
Tissue/cell: mouse spleen tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Stabilin 2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.| Product Name | STAB2 | HARE Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human Stabilin 2 : 2201-2300/2551 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-P |
| IF-P:1:100-1:500 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | STAB2 |
|---|---|
| Gene Synonyms | FEX2 HARE FEEL2 FELL2 FELE-2 SCARH1 |
| Gene Full Name | stabilin 2 |
| Gene Summary | This gene encodes a large, transmembrane receptor protein which may function in angiogenesis, lymphocyte homing, cell adhesion, or receptor scavenging. The protein contains 7 fasciclin, 15 epidermal growth factor (EGF)-like, and 2 laminin-type EGF-like domains as well as a C-type lectin-like hyaluronan-binding Link module. The protein is primarily expressed on sinusoidal endothelial cells of liver, spleen, and lymph node. The receptor has been shown to bind and endocytose ligands such as hyaluronan, low density lipoprotein, Gram-positive and Gram-negative bacteria, and advanced glycosylation end products. Supporting its possible role as a scavenger receptor, the protein has been shown to cycle between the plasma membrane and lysosomes. [provided by RefSeq, Jul 2008] |
| Alternative Names | FEEL2 FELE-2 FELL2 FEX2 HARE SCARH1 STAB2_HUMAN STAB2 FAS1 EGF-like and X-link domain-containing adhesion molecule 2 Fasciclin EGF-like laminin-type EGF-like and link domain-containing scavenger receptor 2 (FEEL-2) Hyaluronan receptor for endocytosis FELL stabilin 2 hyaluronic acid receptor for endocytosis |
| Molecular Weight(MW) | 154/275 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Membrane. |
| SwissProt ID | Q8WWQ8 |
|---|
IF-P
Tissue/cell: mouse spleen tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Stabilin 2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.| Application Notes | IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
