WB
Western blot analysis of anti-PLXDC2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PLXDC2 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PLXDC2 using anti-PLXDC2 antibody.
PLXDC2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of Caco-2 cells using anti-PLXDC2 antibody.
Overlay histogram showing Caco-2 cells stained with Anti-PLXDC2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLXDC2 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.