WB
Positive control:231, Jurkat, MCF-7, 293T cell lysates.
Recommended dilution: 1000-5000.
Gel: 8%SDS-PAGE.
Lysate: 40 μg.
Lane 1-4: 231, Jurkat, MCF-7, 293T cell lysates.
Primary antibody: GBP5 Antibody at
dilution 1/1000.
Secondary antibody: HRP-conjugated
Goat anti rabbit IgG at 1/5000 dilution
Exposure time: 2 minutes.
IHC
Predicted cell location: Cytoplasm.
Positive control: Human tonsil.
Recommended dilution: 50-200.
The image on the left is immunohistochemistry of paraffin
embedded Human tonsil tissue using GBP5 Antibody at
dilution 1/50.