WB
Western Blot. All lanes CD45 antibody at 1:2000. Secondary Goat polyclonal to mouse IgG at 1/50000 dilution. Exposure time:15min.IHC
IHC image of CD45 Monoclonal Antibody diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.ICC/IF
Immunofluorescence staining of Jurkat cells with CD45 Monoclonal Antibody at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).FC
Overlay histogram showing Jurkat cells stained with CD45 Monoclonal Antibody (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.| Product Name | Mouse anti-CD45 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Receptor-type tyrosine-protein phosphatase C protein (24-575AA). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000-1:32000 IHC:1:200-1:600 ICC/IF:1:100-1:300 FC:1:200-1:600 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PTPRC |
|---|---|
| Gene Synonyms | LCA LY5 B220 CD45 L-CA T200 CD45R GP180 IMD105 |
| Gene Full Name | protein tyrosine phosphatase receptor type C |
| Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
| Alternative Names | B220 antibody CD 45 antibody CD45 antibody CD45 antigen antibody CD45R antibody GP180 antibody L-CA antibody LCA antibody Leukocyte common antigen antibody loc antibody Ly-5 antibody LY5 antibody Ly5 homolog of antibody Lyt-4 antibody OTTHUMP00000033813 antibody OTTHUMP00000033816 antibody OTTHUMP00000033817 antibody OTTHUMP00000038574 antibody Protein tyrosine phosphatase receptor type c polypeptide antibody Protein tyrosine phosphatase receptor type C antibody protein tyrosine phosphatase receptor type C antibody Protein tyrosine phosphatase receptor type c polypeptide antibody Ptprc antibody PTPRC_HUMAN antibody Receptor-type tyrosine-protein phosphatase C antibody T200 antibody T200 glycoprotein antibody T200 leukocyte common antigen antibody |
| Molecular Weight(MW) | 148, 132, 143, 141, 139, 136 KDa(Observed band size: 180-250 KDa) |
| Cellular Localization | Cell membrane,Membrane,Synapse. |
| SwissProt ID | P08575 |
|---|
WB
Western Blot. All lanes CD45 antibody at 1:2000. Secondary Goat polyclonal to mouse IgG at 1/50000 dilution. Exposure time:15min.
IHC
IHC image of CD45 Monoclonal Antibody diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
ICC/IF
Immunofluorescence staining of Jurkat cells with CD45 Monoclonal Antibody at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
FC
Overlay histogram showing Jurkat cells stained with CD45 Monoclonal Antibody (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.| Application Notes | WB:1:2000-1:32000 IHC:1:200-1:600 ICC/IF:1:100-1:300 FC:1:200-1:600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
