WB
Western blot analysis of STIM1 using anti-STIM1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of mouse testis tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of mouse testis tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of K562 cells using anti-STIM1 antibody. Overlay histogram showing K562 cells stained with Anti-STIM1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STIM1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG(1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to STIM1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human STIM1 recombinant protein (Position: E42-L599). Human STIM1 shares 98.2% and 98% amino acid (aa) sequence identity with mouse and rat STIM1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STIM1 |
|---|---|
| Gene Synonyms | GOK TAM TAM1 IMD10 STRMK D11S4896E |
| Gene Full Name | stromal interaction molecule 1 |
| Gene Summary | This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Alternative Names | D11S4896E GOK STIM1 stromal interaction molecule 1 |
| Molecular Weight(MW) | 77 kDa(Observed band size: 85-97 kDa) |
| Cellular Localization | Cell membrane,Cytoplasm,Cytoskeleton,Endoplasmic reticulum,Membrane,Microtubule,Sarcoplasmic reticulum. |
| SwissProt ID | Q13586 |
|---|
WB
Western blot analysis of STIM1 using anti-STIM1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of mouse testis tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of mouse testis tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of STIM1 using anti-STIM1 antibody. STIM1 was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-STIM1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of K562 cells using anti-STIM1 antibody. Overlay histogram showing K562 cells stained with Anti-STIM1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STIM1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG(1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
