WB
Western blot analysis of lysates from H-4-II-E cells using STIM1 Rabbit mAb at 1:200000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45 s.WB
Western blot analysis of lysates from COS-7 cells using STIM1 Rabbit mAb at 1:200000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45 s.ICC/IF
Confocal imaging of K-562 cells using STIM1 Rabbit mAb (dilution 1:2000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.IP
Immunoprecipitation of STIM1 from 300 µg extracts of HeLa cells was performed using 0.3 µg of STIM1 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using STIM1 Rabbit mAb at a dilution of 1:20000.| Product Name | STIM1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:10000 - 1:200000 ICC/IF:1:200 - 1:2000 IP:0.3μg - 2 μg antibody for 200 μg - 400 μg extracts of whole cells |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STIM1 |
|---|---|
| Gene Synonyms | GOK TAM TAM1 IMD10 STRMK D11S4896E |
| Gene Full Name | stromal interaction molecule 1 |
| Gene Summary | This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Alternative Names | GOK TAM TAM1 IMD10 STRMK D11S4896E |
| Molecular Weight(MW) | 62 kDa/77 kDa(Observed band size: 85 kDa) |
| Cellular Localization | Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Single-pass type I membrane protein, cytoskeleton, Cortical endoplasmic reticulum, Endoplasmic reticulum, Plasma membrane, Plasma membrane raft, Sarcoplasmic reticulum. |
| SwissProt ID | Q13586 |
|---|
WB
Western blot analysis of lysates from H-4-II-E cells using STIM1 Rabbit mAb at 1:200000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45 s.
WB
Western blot analysis of lysates from COS-7 cells using STIM1 Rabbit mAb at 1:200000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45 s.
ICC/IF
Confocal imaging of K-562 cells using STIM1 Rabbit mAb (dilution 1:2000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
IP
Immunoprecipitation of STIM1 from 300 µg extracts of HeLa cells was performed using 0.3 µg of STIM1 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using STIM1 Rabbit mAb at a dilution of 1:20000.| Application Notes | WB:1:10000 - 1:200000 ICC/IF:1:200 - 1:2000 IP:0.3μg - 2 μg antibody for 200 μg - 400 μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
