WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dilight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Stromal Interaction Molecule 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human breast was stained with anti-Stromal Interaction Molecule 1 rabbit antibody.IHC
Mouse spleen was stained with anti-Stromal Interaction Molecule 1 rabbit antibody.| Product Name | Stromal Interaction Molecule 1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | STIM1 |
|---|---|
| Gene Synonyms | GOK TAM TAM1 IMD10 STRMK D11S4896E |
| Gene Full Name | stromal interaction molecule 1 |
| Gene Summary | This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Alternative Names | Stromal interaction molecule 1 |
| Molecular Weight(MW) | 77 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | Q13586 |
|---|
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dilight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Stromal Interaction Molecule 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human breast was stained with anti-Stromal Interaction Molecule 1 rabbit antibody.
IHC
Mouse spleen was stained with anti-Stromal Interaction Molecule 1 rabbit antibody.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
