WB
Western blot analysis of Aldolase A/B/C on different lysates with Mouse anti-Aldolase A/B/C antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Aldolase A/B/C antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Aldolase A/B/C antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Application: IHC-Fr. Species: Mouse. Site: brain. Sample: Frozen section. Antibody concentration: 1/500. Antigen retrieval: Not required.| Product Name | Aldolase A/B/C Recombinant Antibody - Mouse IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Aldolase C aa 50-100. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IHC:1:5000 IF-F:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ALDOC |
|---|---|
| Gene Synonyms | ALDC |
| Gene Full Name | aldolase, fructose-bisphosphate C |
| Gene Summary | This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008] |
| Alternative Names | ALDC antibody ALDO C antibody aldoc antibody ALDOC_HUMAN antibody Aldolase 3 antibody Aldolase C Fructose bisphosphate antibody Brain type aldolase antibody Brain-type aldolase antibody Fructoaldolase C antibody Fructose 1 6 biphosphate triosephosphate lyase antibody Fructose bisphosphate aldolase C antibody Fructose-bisphosphate aldolase C antibody |
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | cytoskeleton, cytosol, extracellular exosome, extracellular region. |
| SwissProt ID | P09972 |
|---|
WB
Western blot analysis of Aldolase A/B/C on different lysates with Mouse anti-Aldolase A/B/C antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Aldolase A/B/C antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Aldolase A/B/C antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Application: IHC-Fr. Species: Mouse. Site: brain. Sample: Frozen section. Antibody concentration: 1/500. Antigen retrieval: Not required.| Application Notes | WB:1:5000 IHC:1:5000 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
