WB
Western blot analysis of Aldolase A/B/C on different lysates with Rabbit anti-Aldolase A/B/C antibody at 1/2,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: RAW264.7 cell lysate, Lane 3: C6 cell lysate, Lane 4: Human brain tissue lysate, Lane 5: Mouse brain tissue lysate, Lane 6: Mouse pancreas tissue lysate (negative), Lane 7: Rat brain tissue lysate, Lane 8: Rat pancreas tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aldolase A/B/C antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aldolase A/B/C antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of U-87 MG cells labeling Aldolase A/B/C with Rabbit anti-Aldolase A/B/C antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aldolase A/B/C antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.IF-F
Species: Mouse, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:1,000, Antigen retrieval: Not required.IF-F
Species: Rat, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:1,000, Antigen retrieval: Not required.IF-P
Species: Mouse, Site: Cerebral cortex, Sample: Paraffin-embedded section, Antibody concentration: 1:1,000.| Product Name | Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Aldolase C aa 50-100. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-FIF-PIHCWB |
| WB:1:2000-1:5000 IHC:1:10000 ICC/IF:1:100 IF-F:1:1000 IF-P:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ALDOC |
|---|---|
| Gene Synonyms | ALDC |
| Gene Full Name | aldolase, fructose-bisphosphate C |
| Gene Summary | This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008] |
| Alternative Names | ALDC antibody ALDO C antibody aldoc antibody ALDOC_HUMAN antibody Aldolase 3 antibody Aldolase C Fructose bisphosphate antibody Brain type aldolase antibody Brain-type aldolase antibody Fructoaldolase C antibody Fructose 1 6 biphosphate triosephosphate lyase antibody ALDC antibody ALDO C antibody aldoc antibody ALDOC_HUMAN antibody Aldolase 3 antibody Aldolase C Fructose bisphosphate antibody Brain type aldolase antibody Brain-type aldolase antibody Fructoaldolase C antibody Fructose 1 6 biphosphate triosephosphate lyase antibody Fructose bisphosphate aldolase C antibody Fructose-bisphosphate aldolase C antibody |
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | cytoskeleton, cytosol, extracellular exosome, extracellular region |
WB
Western blot analysis of Aldolase A/B/C on different lysates with Rabbit anti-Aldolase A/B/C antibody at 1/2,000 dilution. Lane 1: U-87 MG cell lysate, Lane 2: RAW264.7 cell lysate, Lane 3: C6 cell lysate, Lane 4: Human brain tissue lysate, Lane 5: Mouse brain tissue lysate, Lane 6: Mouse pancreas tissue lysate (negative), Lane 7: Rat brain tissue lysate, Lane 8: Rat pancreas tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Aldolase A/B/C antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Aldolase A/B/C antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of U-87 MG cells labeling Aldolase A/B/C with Rabbit anti-Aldolase A/B/C antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aldolase A/B/C antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
IF-F
Species: Mouse, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:1,000, Antigen retrieval: Not required.
IF-F
Species: Rat, Site: Cerebral cortex, Sample: Frozen section, Antibody concentration: 1:1,000, Antigen retrieval: Not required.
IF-P
Species: Mouse, Site: Cerebral cortex, Sample: Paraffin-embedded section, Antibody concentration: 1:1,000.| Application Notes | WB:1:2000-1:5000 IHC:1:10000 ICC/IF:1:100 IF-F:1:1000 IF-P:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
