WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dilight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SIX2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.ICC/IF
Fluorescence immunohistochemical analysis of Human colon carcinoma| Product Name | SIX2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SIX2 |
|---|---|
| Gene Full Name | SIX homeobox 2 |
| Gene Summary | This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development. [provided by RefSeq, Dec 2008] |
| Alternative Names | SIX2 Homeobox protein SIX2 Sine oculis homeobox homolog 2 |
| Molecular Weight(MW) | 32 kDa(Observed band size: 35 kDa/38kDa) |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9NPC8 |
|---|
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dilight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SIX2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
ICC/IF
Fluorescence immunohistochemical analysis of Human colon carcinoma| Application Notes | WB:1:2000-1:10000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
