WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dilight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Tuberin antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IF-P
Fluorescence immunohistochemical analysis of Human kidney.