WB
Western blot analysis of DDR2 on different lysates with Mouse anti-DDR2 antibody at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Exposure time: 1 minute 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.