IHC
IHC staining of mouse skeletal muscle using MYH1 (N-terminal) Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using MYH1 (N-terminal antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
IF Staining of mouse skeletal muscle using MYH1 (N-terminal) Polyclonal antibody. Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH1 (N-terminal) antibody at dilution of 1:400 and Multi-rAb Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP experiment of mouse skeletal muscle using MYH1 (N-terminal) Polyclonal antibody. IP result of anti-MYH1 (N-terminal) (5ug;1:1000) with mouse skeletal muscle tissue lysate 4000ug.| Product Name | MYH1 (N-terminal) Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MYH1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCIP |
| IHC:1:2000-1:8000 IF-P:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYH1 |
|---|---|
| Gene Synonyms | MYHa HEL71 MYHSA1 MyHC-2x MyHC-2X/D |
| Gene Full Name | HNF1 homeobox B |
| Gene Summary | myosin heavy chain 1 |
| Alternative Names | MYH1 MyHC-2x MyHC-IIx/d Myosin 1 Myosin-1 |
| Molecular Weight(MW) | 1939 aa, 223 kDa(Observed band size: 220-230 kDa) |
| Cellular Localization | Cytoplasm,Thick filament. |
| SwissProt ID | P12882 |
|---|
IHC
IHC staining of mouse skeletal muscle using MYH1 (N-terminal) Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using MYH1 (N-terminal antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
IF Staining of mouse skeletal muscle using MYH1 (N-terminal) Polyclonal antibody. Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH1 (N-terminal) antibody at dilution of 1:400 and Multi-rAb Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP experiment of mouse skeletal muscle using MYH1 (N-terminal) Polyclonal antibody. IP result of anti-MYH1 (N-terminal) (5ug;1:1000) with mouse skeletal muscle tissue lysate 4000ug.| Application Notes | IHC:1:2000-1:8000 IF-P:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
