WB
Western blot analysis of various lysates using TIM-1/KIM-1/HAVCR Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): MEF. Exposure time: 90 s.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using TIM-1/KIM-1/HAVCR Rabbit mAb at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.IP
Immunoprecipitation of TIM-1/KIM-1/HAVCR from 300 µg extracts of A20 cells was performed using 2 µg of TIM 1/KIM-1/HAVCR Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TIM-1/KIM-1/HAVCR Rabbit mAb at a dilution of 1:1000.| Product Name | TIM-1/KIM-1/HAVCR Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:2000 - 1:5000 IHC:1:2000 - 1:8000 IP:0.5 μg - 4 μg antibody for 200 μg - 400 μg extracts of whole cells |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Havcr1 |
|---|---|
| Gene Synonyms | Tim1 KIM-1 TIM-1 Timd1 |
| Gene Full Name | hepatitis A virus cellular receptor 1 |
| Gene Summary | Enables phosphatidylserine binding activity. Acts upstream of or within positive regulation of mast cell activation; response to lipopolysaccharide; and response to wounding. Located in brush border and cell surface. Is expressed in aorta-gonad-mesonephros; brain; genitourinary system; liver; and lung. Human ortholog(s) of this gene implicated in atopic dermatitis. Orthologous to human HAVCR1 (hepatitis A virus cellular receptor 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Tim1 KIM-1 TIM-1 Timd1 |
| Molecular Weight(MW) | 31 kDa/33 kDa(Observed band size: 75 kDa) |
| Cellular Localization | Cell membrane, Cell projection, phagocytic cup, Cytoplasmic vesicle, phagosome. |
| SwissProt ID | Q5QNS5 |
|---|
WB
Western blot analysis of various lysates using TIM-1/KIM-1/HAVCR Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): MEF. Exposure time: 90 s.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using TIM-1/KIM-1/HAVCR Rabbit mAb at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
IP
Immunoprecipitation of TIM-1/KIM-1/HAVCR from 300 µg extracts of A20 cells was performed using 2 µg of TIM 1/KIM-1/HAVCR Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TIM-1/KIM-1/HAVCR Rabbit mAb at a dilution of 1:1000.| Application Notes | WB:1:2000 - 1:5000 IHC:1:2000 - 1:8000 IP:0.5 μg - 4 μg antibody for 200 μg - 400 μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
