WB
Western blot analysis of TIM 1 on different lysates with Rabbit anti-TIM 1 antibody at 1/1,000 dilution. Lane 1: Huh7 cell lysate, Lane 2: HepG2 cell lysate, Lane 3: NCI-H441 cell lysate, Lane 4: Jurkat cell lysate, Lane 5: NIH/3T3 cell lysate, Lane 6: RAW264.7 cell lysate, Lane 7: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | TIM 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human TIM 1 aa 51-100 / 364. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HAVCR1 |
|---|---|
| Gene Synonyms | TIM KIM1 TIM1 CD365 HAVCR KIM-1 TIM-1 TIMD1 TIMD-1 HAVCR-1 |
| Gene Full Name | hepatitis A virus cellular receptor 1 |
| Gene Summary | The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. The protein is a receptor for multiple other viruses, including Ebola virus, Marburg virus, Dengue virus, and Zika virus and is a possible entry factor for SARS-CoV-2 and other coronaviruses. [provided by RefSeq, Sep 2021] |
| Alternative Names | CD365 antibody HAVCR 1 antibody HAVCR antibody HAVcr-1 antibody Havcr1 antibody Hepatitis A virus cellular receptor 1 antibody Kidney injury molecule 1 antibody KIM 1 antibody KIM-1 antibody T cell immunoglobin domain and mucin domain protein 1 antibody CD365 antibody HAVCR 1 antibody HAVCR antibody HAVcr-1 antibody Havcr1 antibody Hepatitis A virus cellular receptor 1 antibody Kidney injury molecule 1 antibody KIM 1 antibody KIM-1 antibody T cell immunoglobin domain and mucin domain protein 1 antibody T cell immunoglobulin mucin family member 1 antibody T cell immunoglobulin mucin receptor 1 antibody T-cell immunoglobulin and mucin domain-containing protein 1 antibody T-cell membrane protein 1 antibody TIM antibody TIM-1 antibody TIM1 antibody TIMD 1 antibody TIMD-1 antibody TIMD1 antibody TIMD1_HUMAN antibody |
| Molecular Weight(MW) | 39kDa(Observed band size: 50kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of TIM 1 on different lysates with Rabbit anti-TIM 1 antibody at 1/1,000 dilution. Lane 1: Huh7 cell lysate, Lane 2: HepG2 cell lysate, Lane 3: NCI-H441 cell lysate, Lane 4: Jurkat cell lysate, Lane 5: NIH/3T3 cell lysate, Lane 6: RAW264.7 cell lysate, Lane 7: PC-12 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
