WB
Western Blot analysis of 293 cells using Phospho-LIMK-1 Polyclonal Antibody diluted at 1:1000.WB
Western Blot analysis of HEPG2 using Phospho-LIMK-1 Polyclonal Antibody. Antibody was diluted at 1:1000.IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using LIMK1 (Phospho-Thr508) Antibody. The picture on the right is blocked with the phospho peptide.| Product Name | LIMK-1 (Phospho Thr508) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human LIMK1 around the phosphorylation site of Thr508. AA range:471-520. |
| Modification | p-Thr508 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LIMK1 |
|---|---|
| Gene Synonyms | LIMK LIMK-1 |
| Gene Full Name | LIM domain kinase 1 |
| Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011] |
| Alternative Names |
LIMK1 LIMK LIM domain kinase 1 LIMK-1 |
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Cytoplasm . Nucleus . Cytoplasm , cytoskeleton . Cell projection , lamellipodium . Predominantly found in the cytoplasm. Localizes in the lamellipodium in a CDC42BPA , CDC42BPB and FAM89B/LRAP25-dependent manner. |
| SwissProt ID | P53667 |
|---|
WB
Western Blot analysis of 293 cells using Phospho-LIMK-1 Polyclonal Antibody diluted at 1:1000.
WB
Western Blot analysis of HEPG2 using Phospho-LIMK-1 Polyclonal Antibody. Antibody was diluted at 1:1000.
IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using LIMK1 (Phospho-Thr508) Antibody. The picture on the right is blocked with the phospho peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
