WB
WB analysis of HeLa using SLC25A15 Polyclonal antibody. HeLa cells were subjected to SDS PAGE followed by western blot with SLC25A15 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.WB
WB analysis of mouse liver using SLC25A15 Polyclonal antibody. mouse liver tissue were subjected to SDS PAGE followed by western blot with SLC25A15 antibody at dilution of 1:200 incubated at room temperature for 1.5 hours.IHC
IHC staining of human brain using SLC25A15 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human brain using SLC25A15 antibody at dilution of 1:50 (under 10x lens).ICC/IF
IF Staining of MCF-7 using SLC25A15 Polyclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | SLC25A15 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SLC25A15 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC25A15 |
|---|---|
| Gene Synonyms | HHH ORC1 ORNT1 LNC-HC D13S327 |
| Gene Full Name | solute carrier family 25 member 15 |
| Gene Summary | This gene is a member of the mitochondrial carrier family. The encoded protein transports ornithine across the inner mitochondrial membrane from the cytosol to the mitochondrial matrix. The protein is an essential component of the urea cycle, and functions in ammonium detoxification and biosynthesis of the amino acid arginine. Mutations in this gene result in hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome. There is a pseudogene of this locus on the Y chromosome.[provided by RefSeq, May 2009] |
| Alternative Names | ORNT1 ORC1 |
| Molecular Weight(MW) | 33 kDa(Observed band size: 30-33 kDa) |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion inner membrane. |
| SwissProt ID | Q9Y619 |
|---|
WB
WB analysis of HeLa using SLC25A15 Polyclonal antibody. HeLa cells were subjected to SDS PAGE followed by western blot with SLC25A15 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB
WB analysis of mouse liver using SLC25A15 Polyclonal antibody. mouse liver tissue were subjected to SDS PAGE followed by western blot with SLC25A15 antibody at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human brain using SLC25A15 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human brain using SLC25A15 antibody at dilution of 1:50 (under 10x lens).
ICC/IF
IF Staining of MCF-7 using SLC25A15 Polyclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SLC25A15 antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:1000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
