IHC
Immunohistochemistry analysis of paraffin-embedded
Human pancreas(positive) and tonsil(negative) tissue
using Trypsin Rabbit mAb at a dilution of 1:5000
(40x lens). High pressure antigen retrieval performed
with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embedded Human
pancreas tissue using Trypsin Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3
conjugated Goat anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclear staining (Blue).
High pressure antigen retrieval performed with 0.01M
Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.