WB
Western blot analysis of lysates from Mouse testis, using Rab11-FIP4 Antibody at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 30s with ECL Kit. Bands result from membrane strip incubation.IHC
Rab11-FIP4 Antibody at 1/100 staining rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.ICC/IF
Rab11-FIP4 Antibody staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to Rab11-FIP4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Rab11-FIP4, corresponding to a region within the internal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAB11FIP4 |
|---|---|
| Gene Synonyms | FIP4-Rab11 RAB11-FIP4 |
| Gene Full Name | RAB11 family interacting protein 4 |
| Gene Summary | The protein encoded by this gene interacts with RAB11 and is thought to be involved in bringing recycling endosome membranes to the cleavage furrow in late cytokinesis. Hypoxic conditions can lead to an upregulation of the encoded protein and enhance the metastatic potential of hepatocellular carcinoma. [provided by RefSeq, Oct 2016] |
| Alternative Names | Rab11 family interacting protein 4 RAB11 FIP4 Rab11 FIP4 like RGD1305063 RP23-271B20.4 |
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Endosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Recycling endosome membrane>Peripheral membrane protein. Cleavage furrow. Midbody. Cytoplasmic vesicle. |
| SwissProt ID | Q86YS3 |
|---|
WB
Western blot analysis of lysates from Mouse testis, using Rab11-FIP4 Antibody at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 30s with ECL Kit. Bands result from membrane strip incubation.
IHC
Rab11-FIP4 Antibody at 1/100 staining rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
ICC/IF
Rab11-FIP4 Antibody staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:1000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
